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Reagent

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From Wikipedia, the free encyclopedia
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  1. Reagent
Reagent
Substance added to a system to cause a chemical reaction

In chemistry, a reagent (/riˈeɪdʒənt/ ree-AY-jənt) or analytical reagent is a substance or compound added to a system to cause a chemical reaction, or test if one occurs.1 A reactant is a substance or compound that is consumed in a chemical reaction.1 The terms reactant and reagent are often used interchangeably, but reactant specifies a substance consumed in the course of a chemical reaction; reagent is used in the context of chemical analysis, while reactant is used in the context of reaction itself. Solvents, though involved in the reaction mechanism, are usually not called reactants. Similarly, catalysts are not consumed by the reaction, so they are not reactants. In biochemistry, especially in connection with enzyme-catalyzed reactions, the reactants are commonly called substrates.

"Reactants" redirects here; not to be confused with Reactance or Regent.

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Contents
  • (Top)
  • Definitions
  • Commercial or laboratory preparations
  • Biology
  • See also
  • References
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Contents
Contents8 sections
  • (Top)
  • Definitions
  • Organic chemistry
  • Analytical chemistry
  • Commercial or laboratory preparations
  • Biology
  • Tool compounds
  • See also
  • References
  • External links
  • Recent changes
  • Rabbit hole
Study aids for Reagent

Terminology

analytical reagent
In chemistry, a reagent (/riˈeɪdʒənt/ ree-AY-jənt) or analytical reagent is a substance or compound added to a system to cause a chemical reaction, or test if one occurs.
reactant
A reactant is a substance or compound that is consumed in a chemical reaction.
reagent-grade
In commercial or laboratory preparations, reagent-grade designates chemical substances meeting standards of purity that ensure the scientific precision and reliability of chemical analysis, chemical reactions or physical testing.

Terms the article defines in bold, quoted in context.

Categories:
  • Reagents
  • Reagents for organic chemistry
  • Biological techniques and tools
  • Chemical reactions
  • Reagents for biochemistry

471 words · 12 references · revision 1369246191 · CC BY-SA 4.0

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Key concepts

  • Chemical analysis2
  • Sulfur1
  • Chemistry1
  • Compound (chemistry)1
  • Chemical reaction1
  • Solvent1
  • Reaction mechanism1
  • Catalysis1
  • Biochemistry1
  • Enzyme1

The articles this one links to most, weighted towards the introduction.

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Definitions

Organic chemistry

In organic chemistry, the term "reagent" denotes a chemical ingredient (a compound or mixture, typically of inorganic or small organic molecules) introduced to cause the desired transformation of an organic substance. Examples include the Collins reagent, Fenton's reagent, and Grignard reagents.2

Analytical chemistry

In analytical chemistry, a reagent is a compound or mixture used to detect the presence or absence of another substance, e.g. by a color change, or to measure the concentration of a substance, e.g. by colorimetry. Examples include Fehling's reagent, Millon's reagent, and Tollens' reagent.3

Commercial or laboratory preparations

In commercial or laboratory preparations, reagent-grade designates chemical substances meeting standards of purity that ensure the scientific precision and reliability of chemical analysis, chemical reactions or physical testing. Purity standards for reagents are set by organizations such as ASTM International or the American Chemical Society. For instance, reagent-quality water must have very low levels of impurities such as sodium and chloride ions, silica, and bacteria, as well as a very high electrical resistivity. Laboratory products which are less pure, but still useful and economical for undemanding work, may be designated as technical, practical, or crude grade to distinguish them from reagent versions.4

Biology

In the field of biology, the biotechnology revolution in the 1980s grew from the development of reagents that could be used to identify and manipulate the chemical matter in and on cells.56 These reagents included antibodies (polyclonal and monoclonal), oligomers, all sorts of model organisms and immortalised cell lines, reagents and methods for molecular cloning and DNA replication, and many others.67

Tool compounds

Tool compounds are an important class of reagent in biology. They are small molecules or biochemicals like siRNA or antibodies that are known to directly interact with or modulate a specific biomolecule—for example a drug target—but are unlikely to be useful as drugs themselves, and are often starting points in the drug discovery process.89

However, many natural substances are hits in almost any assay in which they are tested, and therefore not useful as tool compounds. Medicinal chemists class them instead as . One example is .

See also

  • Limiting reagent
  • Common reagents
  • Product
  • Reagent bottle
  • Substrate
  • All pages with titles containing Reagent

References

  1. 1IUPAC, Compendium of Chemical Terminology, 5th ed. (the "Gold Book") (2025). Online version: (1996) "Reactant". doi:10.1351/goldbook.R05163
  2. 2"Common Reagents in Organic Chemistry". CommonOrganicChemistry.com. Retrieved 9 March 2026.
  3. 3"Analytical Reagents - Analytical Products / Alfa Chemistry". Alfa Chemistry. Retrieved 9 March 2026.
  4. 4"Preparing Chemical Solutions, Reagents, and Buffers". St. Olaf College. Retrieved 9 March 2026.
  5. 5Fox, Jeffrey L (1 January 1979). "Antibody reagents revolutionizing immunology". Chemical & Engineering News Archive. 57: 15–17. doi:10.1021/cen-v057n001.p015.
  6. 6. NIH. June 4, 1998. Archived from on August 16, 2000.

External links

  • Media related to Reagents at Wikimedia Commons
  • The dictionary definition of reactant at Wiktionary
pan-assay interference compounds
curcumin
10
11
12
"Report of the National Institutes of Health (NIH) Working Group on Research Tools"
the original
  • 7Ishino, S; Ishino, Y (29 August 2014). "DNA polymerases as useful reagents for biotechnology: the history of developmental research in the field". Frontiers in Microbiology. 5: 465. doi:10.3389/fmicb.2014.00465. PMC 4148896. PMID 25221550.
  • 8Kenakin, T; Bylund, DB; Toews, ML; Mullane, K; Winquist, RJ; Williams, M (1 January 2014). "Replicated, replicable and relevant-target engagement and pharmacological experimentation in the 21st century". Biochemical Pharmacology. 87 (1): 64–77. doi:10.1016/j.bcp.2013.10.024. PMID 24269285.
  • 9Lindsley, CW (25 September 2014). "2013 Philip S. Portoghese Medicinal Chemistry Lectureship: drug discovery targeting allosteric sites". Journal of Medicinal Chemistry. 57 (18): 7485–7498. doi:10.1021/jm5011786. PMC 4174999. PMID 25180768.
  • 10Baker, Monya (9 January 2017). "Deceptive curcumin offers cautionary tale for chemists". Nature. 541 (7636): 144–145. Bibcode:2017Natur.541..144B. doi:10.1038/541144a. PMID 28079090.
  • 11Dahlin, JL; Walters, MA (July 2014). "The essential roles of chemistry in high-throughput screening triage". Future Medicinal Chemistry. 6 (11): 1265–1290. doi:10.4155/fmc.14.60. PMC 4465542. PMID 25163000.
  • 12Baell, JB; Holloway, GA (8 April 2010). "New substructure filters for removal of pan assay interference compounds (PAINS) from screening libraries and for their exclusion in bioassays". Journal of Medicinal Chemistry. 53 (7): 2719–2740. doi:10.1021/jm901137j. PMID 20131845.
  • Created
    18 Feb 2003
    History
    445 edits
    307 contributors
    No edits in the last 30 days
    Last edited
    13 Aug 2026

    445 revisions in total · 307 contributors

    1. 13 Aug 2026, 19:56 UTCTrappist the monkmremoved deprecated |citeseerx= parameters;−27diff
    2. 19 May 2026, 16:48 UTCAndrewPeterTcombine hatnotes−16diff
    3. 15 Mar 2026, 18:04 UTCCassiusClayAndAlizemWikilinks added+25diff
    4. 9 Mar 2026, 17:19 UTCS1r Gawa1n 2004§ Tool compounds Extraction of {{ambiguous|reason=needs punctuating to clarify phrase structure|date=February 2024}} due to rectification of the issue pertaining.−54diff
    5. 9 Mar 2026, 17:14 UTCS1r Gawa1n 2004§ Commercial or laboratory preparations+340diff
    6. 9 Mar 2026, 16:31 UTCS1r Gawa1n 2004§ Organic chemistry−21diff
    7. 9 Mar 2026, 16:31 UTCS1r Gawa1n 2004§ Definitions0diff
    8. 9 Mar 2026, 16:31 UTCS1r Gawa1n 2004§ Definitions+451diff

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    Created
    18 Feb 2003
    History
    445 edits
    307 contributors
    No edits in the last 30 days
    Last edited
    13 Aug 2026

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